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“哈靈生物”產品文獻:miR-4504參與三角帆蚌珍珠層顏色的形成

熱烈祝賀 上海海洋大學 -陳夏君 陳老師 文獻發表成功

文獻主題:

miR-4504參與三角帆蚌珍珠層顏色的形成

文獻中引用的我司產品(加亮部分):

Mantle samples (500mg middle mantle and 500mg fringe

mantle from each mussel) were collected from groups A, N and P.

Samples were immediately frozen in liquid nitrogen and stored

at 80 C until use. Tyrosinase activity was measured using the

Tissue Tyrosinase Activity Assay Kit (HaLing, Shanghai, China), according

to the manufacturer's instructions. Mantle samples were

comminuted in liquid nitrogen and treated with lysis buffer. After

centrifugation, the supernatant was collected and mixed with

buffer and tyrosine. All samples were incubated at 25 C for 1 h.

Tyrosinase activity was measured using a microplate reader with

the dopachrome content at 475 nm (UV-3010; Shimadzu, Japan).

One-way ANOVAs were performed using SPSS 17.0 to determine

significant differences between groups. Data from the tyrosinase

activity experiments were expressed as the mean ± SD (n ¼ 6). A pvalue

of <0.05 was considered statistically significant.

文獻鏈接:

鏈接: https://pan.baidu.com/s/1XoZQb0hjYlsTIV4aE1Zt-Q 提取碼: ps5k

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