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“哈靈生物”產品文獻:三角帆蚌的HcTyr和HcTyp-1

 熱烈祝賀 上海海洋大學 -陳夏君 陳老師 文獻發表成功

文獻主題:

三角帆蚌的HcTyr和HcTyp-1

文獻中引用的我司產品(加亮部分):

Themantle samples (0.5 g of themantle center and 0.5 g of themantle

pallial and mantle edge from each sample) from eight healthy

H. cumingii specimens (four purple mussels and four white mussels)

were collected and immediately frozen in liquid nitrogen and stored

at−80 °C until use. The tyrosinase activity was estimated using the tissue

tyrosinase activity assay kit (HaLing, Shanghai, China) according to

在−80°C下使用。酪氨酸酶活性用組織學方法測定

酪氨酸酶活性測定試劑盒(上海哈靈生物科技有限公司)

themanufacturer's instructions. In brief, the sampleswere comminuted

in some liquid nitrogen with a mortar and pestle and treated with the

lysis buffer. The solution was centrifuged, and the supernatant was collected

andmixed with buffer and substrate (tyrosine). Finally, the samples

were incubated at 25 °C for 60 min, and tyrosinase activity was

measured using a spectrophotometer with the dopachrome content at

475 nm (UV-3010; Shimadzu, Japan). Tyrosinase catalyzes a reaction

that converts tyrosine to dopachrome by hydroxylation and then to

dopaquinone. One unit of tyrosinase activitywas defined as the amount

of activity that produced 1 μmol of dopachrome per minute via the hydroxylation

of tyrosine by tyrosinase at 25 °C. One-way ANOVAwas performed

using SPSS 17.0 to determine whether there were any

significant differences. Data from the TYR activity experiments were

expressed as the mean± SD (n=5) values. Significance was accepted

at the level of p b 0.05.

文獻鏈接:

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